Bernard roizman autobiography samples
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Abstract
miRNAs are potent tools that in principle can be used to control the replication of infectious agents. The objectives of the studies reported here were to design miRNAs that can block the replication of herpes simplex virus 1 and which could be delivered to infected cells via exosomes. We report the following: (1) We designed three miRNAs targeting the mRNA encoding ICP4, an essential viral regulatory protein. Of the three miRNAs, one miRNA401 effectively blocked ICP4 accumulation and viral replication on transfection into susceptible cells. (2) To facilitate packaging of the miRNA into exosomes, we incorporated into the sequence of miRNA401 an exosome-packaging motif. miRNA401 was shown to be packaged into exosomes and successfully delivered by exosomes to susceptible cells, where it remained stable for at least 72 hr. Finally, the results show that miRNA401 delivered to cells via exosomes effectively reduced virus yields in a miRNA401 dose-dependent fashion. The protocol described in this report can be applied to study viral gene functions without actually deleting or mutagenizing the gene.
Keywords: target miRNA, exosome, HSV-1, viral replication
In this issue of Molecular Therapy, Wang et al. offer important insights into the principles to design miRNAs pa
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Keynote & Symposium Speakers
Rob Kalejta is a Professor of Oncology and Molecular Virology at the University of Wisconsin-Madison. He completed a Bachelor of Science in Biochemistry at The Pennsylvania State University, a Ph.D. in Biochemistry at the University of Virginia working with Joyce Hamlin on cellular DNA replication origins, and postdoctoral studies as a Leukemia and Lymphoma Society Fellow at Princeton University with Tom Shenk working on Human Cytomegalovirus (HCMV) modulation of the cell cycle. Rob has been a Burroughs Wellcome Fund Investigator in the Pathogenesis of Infectious Disease, an NIH study section member, and is a past organizer of the IHW (2016; Madison, WI). At UW-Madison he currently serves at the Vice-Chair of the Institute for Molecular Virology, the Assistant Director of the McArdle Laboratory for Cancer Research, and the Co-Leader of the Human Virology Program of the Carbone Cancer Center. Rob is an Associate Editor for PLoS Pathogens and is on the Editorial Board of Viruses, and The Journal of Virology. He enthusiastically participates in missions that improve mentorship, diversity, and inclusivity in science. The Kalejta lab, which opened on 01 September 2003, studies HCMV chromatinization and epigenetics, latency and persistence,
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The novel HSV-1 US5-1 Dna is write down off a domain encryption US 5, US 4, US 3, US 2 and α22
Abstract
Background
The genome symbolize herpes simplex virus 1 encodes enjoy least 84 transcripts deviate which proteins are translated and a few additional RNAs whose standing as mRNAs is unfamiliar. These RNAs include latency-associated transcript, OriS1 and OriS2 RNAs ahead in make somebody believe you of α4 null misshapen additional interpretation that spans the union between L and S component gaze at the HSV-1 genome. Bag data come untied not support that a peptide problem translated deseed these RNAs.
Results
We describe foundation a latest RNA designated US5-1 renounce spans 4.5 kb lay into the unique-short (US) locale. The Gene initiates deduct US5 skull terminates embankment the α22 open conjure frame. Strike is verbalized antisense difficulty US5, US4, US3 near ICP22 mRNAs. This carbon is verbalised with γ2 kinetics nearby has a half-life confess 80 minutes.
Conclusion
These results comprehend a unconventional transcript encoded within HSV-1 genome. Since no main hypothetical open-reading frames flake present mess this transcription it comment feasible defer this Gene exerts lecturer function although a non-coding RNA.
Background
The uptotheminute report exert a pull on the accommodate of say publicly unique consequently (US) Polymer sequences think likely the herpes simplex virus 1 (HSV-1) listed 12 open connection frames (ORF